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Assembly–disassembly driven “off–on” fluorescent perylene bisimide probes for detecting and tracking of proteins in living cells

Author:
Bo, Fuli, Gao, Baoxiang, Duan, Wenfeng, Li, Hongxia, Liu, Hongmei, Bai, Qianqian
Source:
RSC advances 2013 v.3 no.38 pp. 17007-17010
ISSN:
2046-2069
Subject:
aqueous solutions, fluorescence, fluorescent dyes, nanomaterials, proteins
Abstract:
We have developed an assembly–disassembly driven “off–on” fluorescent bioprobe based on amphiphilic perylene bisimide. In aqueous solutions, the amphiphilic probes can form self-assembled nanostructures with quenched fluorescence. These probes could then be disassembled by the binding of the probes to the targeted proteins, and the disassembly process leads to a 23-fold enhancement in fluorescence intensity. Amphiphilic perylene bisimides could be used as probes for the detection and tracking of targeted proteins under live cell conditions.
Agid:
6645563