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Distribution and Transformation of Soluble Carbohydrates during Germination Growth of Sorghum
- Newton, R. J., Baltuskonis, D. A., Goeschl, J. D., Meckenstock, D. H., Miller, F. R.
- Crop science 1980 v.20 no.2 pp. 265-268
- Sorghum bicolor, dhurrin, endosperm, fructose, germination, glucose, roots, seedlings, shoots, sucrose
- Soluble components of sorghum (Sorghum bicolor (L.) Moench) seedlings growing in distilled water-sand media were quantified with gas-liquld-chromatography. In light, peak levels of soluble component accumulation occurred in the roots in 4 days, in the endosperm in 8 days, and in the shoots in 10 days. Sucrose (2 µg/mg) was the predominant component in the dry seed. In 4-day-old seedlings, glucose and fructose levels were 15 and 20 µg/mg, respectively, in both shoots and roots. At l0 days, dhurrin levels in the shoot were 35 to 40 µg/mg and comprised 51% of the total soluble component while glucose accounted for 21%. In the dark, dhurrin levels in 10-day-old shoots were 27% of the total solubles and glucose levels were 45%.. Dhurrin levels were highest in the coleoptile-shoot primordium region (90 µg/mg) and the upper, growing portion of the mesocotyl (28 µg/mg). Monosaccharide levels were highest in the upper, mesocotyl region with glucose at 74 µg/mg and fructose at 51 µg/mg. In 4-day-old scutella, sucrose to glucose ratios were greater than 3. Dhurrin and monosaccharide levels were reciprocally related; this indicated the precursor roles of both glucose and fructose in glycoside synthesis. The detection of a glycoside with gas-liquid-chromatography has several distinct advantanges over the traditional, enzymatic-colorimetric assay.