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Overexpression of iron superoxide dismutase in transformed poplar modifies the regulation of photosynthesis at low CO2 partial pressures or following exposure to the prooxidant herbicide methyl viologen

Author:
Arisi, A.C.M., Cornic, G., Jouanin, L., Foyer, C.H.
Source:
Plant physiology 1998 v.117 no.2 pp. 565-574
ISSN:
0032-0889
Subject:
Populus tremula, hybrids, Populus alba, leaves, photosynthesis, net assimilation rate, light, carbon dioxide, pressure, oxidants, herbicides, hydrogen peroxide, glutathione, ascorbic acid, gene transfer, genetic transformation, Arabidopsis thaliana, chloroplasts, iron, superoxide dismutase, peroxidase, glutathione reductase (NADPH), dehydroascorbic acid, oxidoreductases, enzyme activity, transgenic plants, gene expression, chemical constituents of plants, stress response
Abstract:
Chloroplast-targeted overexpression of an Fe superoxide dismutase (SOD) from Arabidopsis thaliana resulted in substantially increased foliar SOD activities. Ascorbate peroxidase, glutathione reductase, and monodehydroascorbate reductase activities were similar in the leaves from all of the lines, but dehydroascorbate reductase activity was increased in the leaves of the FeSOD transformants relative to untransformed controls. Foliar H2O2, ascorbate, and glutathione contents were comparable in all lines of plants. Irradiance-dependent changes in net CO2 assimilation and chlorophyll a fluorescence quenching parameters were similar in all lines both in air (21% O2) and at low (1%) O2. CO2-response curves for photosynthesis showed similar net CO2-exchange characteristics in all lines. In contrast, values of photochemical quenching declined in leaves from untransformed controls at intercellular CO2 (Ci) values below 200 microliters L-1 but remained constant with decreasing Ci in leaves of FeSOD transformants. When the O2 concentration was decreased from 21 to 1%, the effect of FeSOD overexpression on photochemical quenching at limiting Ci was abolished. At high light (1000 micromol m-2 s-1) a progressive decrease in the ratio of variable (F(v)) to maximal (F(m)) fluorescence was observed with decreasing temperature. At 6 degrees C the high-light-induced decrease in the F(v)/F(m) ratio was partially prevented by low O2 but values were comparable in all lines. Methyl viologen caused decreased F(v)/F(m) ratios, but this was less marked in the FeSOD transformants than in the untransformed controls. These observations suggest that the rate of superoxide dismutation limits flux through the Mehler-peroxidase cycle in certain conditions.
Agid:
665360