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pH-responsive hybrid magnetic polyelectrolyte complex based on alginate/BSA as efficient nanocarrier for curcumin encapsulation and delivery
- Amani, Soheil, Mohamadnia, Zahra, Mahdavi, Atiyeh
- International journal of biological macromolecules 2019 v.141 pp. 1258-1270
- Fourier transform infrared spectroscopy, X-ray diffraction, alginates, antineoplastic activity, antineoplastic agents, atomic force microscopy, bovine serum albumin, breast neoplasms, chlorides, curcumin, cytotoxicity, electrolytes, electrostatic interactions, encapsulation, equations, iron oxides, nanocarriers, nanoparticles, neoplasm cells, pH, phosphates, polymers, scanning electron microscopy, thermogravimetry, ultraviolet-visible spectroscopy, zeta potential
- Curcumin-loaded hybrid magnetic nanoparticles (HMNPs) were formulated using a simple and green method. At first, surface of Fe₃O₄ nanoparticles was modified using alginate (ALG), and magnetic ALG/Fe₃O₄ nanoparticles with a size of 33 nm and a zeta potential of -25 mv were prepared. Then, a complex including bovine serum albumin (BSA) and poly((3-acrylamidopropyl)trimethylammonium chloride) (p(APTMACl)) was prepared and optimized. The behavior of the BSA/p(APTMACl) complex was evaluated as a function of pH and protein/polymer ratio by UV–Vis spectroscopy, zeta potential, and DLS analysis. Finally, the hybrid complex of BSA/p(APTMACl)@ALG/Fe₃O₄ were prepared using mixing of the BSA/p(APTMACl) and ALG/Fe₃O₄ systems with opposite charges via electrostatic interactions. The prepared BSA/p(APTMACl) @ALG/Fe₃O₄ complex was named hybrid magnetic nanoparticles (HMNPs) and characterized by UV–Vis spectroscopy, SEM, FT-IR, TGA, XRD, VSM, and AFM. Three samples including pure Fe₃O₄, ALG/Fe₃O₄, and the final HMNPs exhibited no cytotoxic effect on MCF-7 breast cancer cell using the MTT assay. MTT assay to test the antitumor activity of bare and encapsulated-curcumin against MCF-7 cells showed BSA/p(APTMACl)@ALG/Fe₃O₄ nanoparticles are able to stabilize the curcumin anti-cancer drug and also enhancing the antitumor activity of the encapsulated curcumin. After loading of curcumin with a high efficiency of 95%. The accumulative release profiles of curcumin were investigated at 37 °C in phosphate buffered saline (PBS, pH 3 and 7.4). The release of curcumin-loaded HMNPs followed the Korsmeyer-Peppas equation and anomalous (non-fickian) diffusion mechanism at pHs 3 and 7.4.