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miR-21 targets jnk and ccr7 to modulate the inflammatory response of grass carp following bacterial infection

Tao, Lizhu, Xu, Xiaoyan, Fang, Yuan, Wang, Anqi, Zhou, Fenglin, Shen, Yubang, Li, Jiale
Fish & shellfish immunology 2019 v.94 pp. 258-263
Aeromonas hydrophila, Ctenopharyngodon idella, bacterial infections, death, fish, gene overexpression, genes, inflammation, interleukin-12, interleukin-1beta, interleukin-6, luciferase, microRNA, septic shock, tumor necrosis factor-alpha
Grass carp septicemia is a systemic inflammatory response that develops following a bacterial infection. The hyperinflammatory state develops could lead to septic shock and lethality. There is increasing evidence that microRNAs are involved in the regulation of the inflammatory response. In the present study, miR-21 was confirmed to be involved in the inflammatory response following infection with Aeromonas hydrophila and LPS stimulation. Both jnk and ccr7 were identified as target gene of miR-21 by overexpression, inhibition, and dual luciferase reporter assays experiments. Meanwhile, miR-21 targets the jnk and ccr7 to modulate downstream pro-inflammatory factors tnf-α, il-1β, il-6, and il-12. Our results provide a theoretical basis for exploring the molecular mechanism of grass carp miR-21 regulating inflammation.