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Nitrogen-15-Depleted versus Nitrogen-15-Enriched Ammonium Sulfate as Tracers in Nitrogen Uptake Studies
- Edwards, A. P., Hauck, R. D.
- Soil Science Society of America journal 1974 v.38 no.5 pp. 765-767
- ammonium sulfate, fertilizers, greenhouse experimentation, nitrogen, plant tissues, spectrometers, stable isotopes, tracer techniques
- Fertilizer particles were prepared from ammonium sulfate with a lower (0.031) than natural (0.366) atom percentage of ¹⁵N. The ¹⁵N-depleted material was compared with ammonium sulfate of a comparable level of ¹⁵N enrichment (0.734 atom %) in a greenhouse pot experiment which measured plant recovery of applied N. There was no statistical difference in N recovery values for five harvests taken over an 18-week period at three rates of applied tracer N, with on exception. Accurate and precise measurement of tracer N in plant tissue was obtained for both ¹⁵N-depleted and ¹⁵N-enriched materials, using two mass spectrometers with different measurement and rcording systems. A nontracer technique gave lower first harvest and higher combined harvest values of percentage recovery of applied N than either tracer technique.