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Organic Matter Study of Whole Soil Samples Using Laser-Induced Fluorescence Spectroscopy

Author:
Milori, Débora Marcondes Bastos Pereira, Galeti, Helder Vinicius Avanço, Martin-Neto, Ladislau, Dieckow, Jeferson, González-Pérez, Martha, Bayer, Cimélio, Salton, Júlio
Source:
Soil Science Society of America journal 2006 v.70 no.1 pp. 57-63
ISSN:
0361-5995
Subject:
Oxisols, carbon, cerrado, conventional tillage, electron paramagnetic resonance spectroscopy, fluorescence, fluorescence emission spectroscopy, homogenization, humic acids, humification, indigenous species, iron, long term experiments, no-tillage, nuclear magnetic resonance spectroscopy, plant residues, soil organic matter, soil sampling, stable isotopes
Abstract:
Fluorescence spectroscopy relies on the fluorescence emitted by rigid conjugated systems and thus can be used to assess the soil organic matter (SOM) humification. This technique is generally applied to solution samples of humic substances, and so far no information exists about its applicability to whole untreated soil samples. The laser-induced fluorescence (LIF) spectroscopy is proposed as a novel technique to assess the organic matter humification in whole soil samples. We sampled the 0- to 2.5-, 2.5- to 5-, 5- to 10-, 10- to 15-, and 15- to 20-cm layers of three Oxisols of long-term experiments located in two sites of the Brazilian Cerrado. The humification index based on LIF spectroscopy (HLIF) of whole soil samples showed a close correlation with the humification indexes A₄/A₁, I₄₆₅/I₃₉₉, and A₄₆₅ obtained after fluorescence spectroscopy analysis of the dissolved humic acids. The HLIF in soils under native cerrado or subjected to no-tillage increased from the top to the deepest layer, which is consistent with the deposition of labile organic matter from plant residues on the soil surface. The soils subjected to conventional tillage, however, showed relatively constant HLIF along the profile, possibly because homogenization imparted by disturbance of the arable layer. Accordingly, for the two top layers, the soils under no-tillage showed a lower HLIF than for conventionally tilled soils. Laser-induced fluorescence spectroscopy is a promising technique to assess humification in whole soil samples, particularly in Oxisols, which due to high concentration of Fe³⁺ are not feasible to electron spin resonance (ESR) and Carbon-13 nuclear magnetic resonance (¹³C NMR) spectroscopy, unless previous treatment is employed.
Agid:
6682614