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Broth rheology and morphological analysis of Solanum chrysotrichum cultivated in a stirred tank
- Trejo-Tapia, G., Jimenez-Aparicio, A., Villarreal, L., Rodriguez-Monroy, M.
- Biotechnology letters 2001 v.23 no.23 pp. 1943-1946
- bioreactors, plant morphology, image analysis
- Solanum chrysotrichum cell cultures were grown in a stirred tank bioreactor and their rheological and morphological behaviour were evaluated. The culture broths exhibited non-Newtonian and shear-thinning characteristics. Pseudoplasticity of the broths was governed by their biomass concentration. The roundness of aggregates measured as the elliptical form factor (EFF) hadimportant changes. At the beginning of the culture the aggregates with an EFF lower than 2 represented 52% of the population, but in stationary phase the proportion increased to 77%. Whereas the size of aggregates did not change 80% of the population had an area lower than 0.1 mm(2). Overall, these results indicate that the shape of the aggregate therefore needs to be considered when studying plant broth rheology.