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Organic degrading bacteria and nitrifying bacteria stimulate the nutrient removal and biomass accumulation in microalgae-based system from piggery digestate

Wang, Min, Zhang, Shu-Chi, Tang, Qin, Shi, Ling-Dong, Tao, Xing-Ming, Tian, Guang-Ming
The Science of the total environment 2019
Cellvibrio, Comamonas, Desmodesmus, Dyadobacter, Flavobacterium, Microbacterium, Paenibacillus, Sphingobacterium, ammonium nitrogen, biodegradation, biomass production, chlorophyll, high-throughput nucleotide sequencing, microalgae, microbial communities, nitrate nitrogen, nitrifying bacteria, nitrite nitrogen
The microalgae-based system has been applied in anaerobic digestate treatment for nutrient removal and biomass production. To optimize its performance in treating piggery digestate, here, commercial bacterial agents, including organic degrading bacteria (Cb) and nitrifying bacteria (Nb), were inoculated into the microalgae-based system dominated by Desmodesmus sp. CHX1 (D). Reactor DN (inoculated with D and Nb) and DCN (inoculated with D, and Cb to Nb at a ratio of 1:2) have better performance on NH4+-N removal, with a final efficiency at 40.26% and 39.87%, respectively, and no NO3−-N or NO2−-N accumulations. The final total chlorophyll concentration, an indicator of microalgal growth, reached 4.74 and 5.47 mg/L in DN and DCN, respectively, three times more than that in D. These results suggested that high NH4+-N removal was achieved by the assimilation into high microalgal biomass after the inoculation with functional bacteria. High-throughput sequencing showed that the richness of microbial community decreased but the evenness increased by inoculating functional microorganisms. Microalgae aggregating bacteria were Cellvibrio, Sphingobacterium, Flavobacterium, Comamonas, Microbacterium, Dyadobacter, and Paenibacillus. This study revealed that the inoculation with functional bacteria reconstructed the microbial community which benefited for the microalgal growth and nutrient removal, providing a promising strategy for treating highly-concentrated digestate.