Main content area

Novel protein kinase C participates catecholamine biosynthesis and immunocompetence modulation in haemocytes of Litopenaeus vannamei

Chang, Zhong-Wen, Chang, Chin-Chyuan
Developmental and comparative immunology 2020 v.103 pp. 103504
Litopenaeus vannamei, biosynthesis, dopamine, dopamine beta-monooxygenase, enzyme activity, genes, glutathione peroxidase, granulocytes, hemocytes, immune response, immunocompetence, monophenol monooxygenase, norepinephrine, phagocytosis, protein kinase C, respiratory burst, shrimp, superoxide dismutase, tyrosine, tyrosine 3-monooxygenase
The catecholamine biosynthesis is required for physiological and immunological responses against stress, and the neuroendocrine-immune regulatory network plays a crucial role in immunocompetence of shrimp. A novel protein kinase C of Litopenaeus vannamei (LvnPKC) is involved in immune defense and signaling transduction in haemocytes, and in the present study, the gene silence technique is conducted to identify the role of LvnPKC on catecholamine biosynthesis and immunocompetence modulation in haemocytes of L. vannamei. The results show that tyrosine significantly increases in haemocytes of LvnPKC-silenced shrimp, and in the meantime, the obvious decrease of L-3, 4-dihydroxyphenylalanine and increase of dopamine as well as the consistent norepinephrine levels are detected. Tyrosine hydroxylase and dopamine β-hydroxylase activities are significantly reduced in haemocytes of LvnPKC-silenced shrimp. Total haemocyte count, hyaline cells and granulocytes insignificantly differ among treatments, and the obvious increase of phenoloxidase activity, respiratory bursts, superoxide dismutase and glutathione peroxidase activities are observed in haemocytes of LvnPKC-silenced shrimp, and furthermore, the downregulated phagocytic activity was observed. It is therefore concluded that the LvnPKC mediates catecholamine biosynthesis and immunocompetence in haemocytes, and plays a crucial role in the neuroendocrine-immune regulatory network.