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Effect of three commercial extenders on sperm motility and fertility in liquid ram semen stored at 15 °C or 5 °C

Arando, Ander, Delgado, Juan Vicente, León, José Manuel, Nogales, Sergio, Navas-González, Francisco Javier, Pizarro, María Gabriela, Pérez-Marín, Carlos Carmelo
Acta veterinaria Hungarica 2019 v.67 no.3 pp. 430-444
egg yolk, lecithins, liquids, male fertility, pregnancy rate, rams, semen, semen extenders, skim milk, soybeans, sperm motility, spermatozoa, storage temperature
The effect of different extenders on sperm motility and fertility was evaluated during liquid storage of ram semen at 5 °C and 15 °C. The semen was collected, pooled and diluted in three commercial extenders: Inra 96® (INRA) based on skimmed milk, Biladyl® A fraction (BIL) based on egg yolk, and Ovixcell® (OVIX) based on soybean lecithin. Then, sperm motility was evaluated at 0, 6, 24, 48, 72 and 96 h. In order to evaluate fertility, samples stored at 15 °C were used after dilution in INRA and OVIX. Results showed that progressive motility was significantly higher up to 72 h of storage in sperm samples maintained at 5 °C in comparison with 15 °C, similarly for each tested diluent. When samples were stored at 5 °C in OVIX, kinematic parameters such as velocity (except curvilinear velocity, VCL), trajectory [linearity (LIN), straightness (STR), wobble (WOB)], amplitude of lateral head displacement (ALH) and beat/cross frequency (BCF) were higher than in INRA and BIL. No significant differences in pregnancy rate were detected between INRA (62.6%) and OVIX (58.9%). In conclusion, liquid storage at 5 °C with OVIX extender is an interesting option since non-animal components are used, and this extender offers similar in vitro and in vivo efficacy as other extenders containing animal components.