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Bisphenol A triggers axonal injury and myelin degeneration with concomitant neurobehavioral toxicity in C57BL/6J male mice

Author:
Khan, Jasim, Salhotra, Shikha, Goswami, Poonam, Akhter, Juheb, Jahan, Shagufta, Gupta, Sakshi, Sharma, Shikha, Banerjee, Basu Dev, Parvez, Suhel, Gupta, Sarika, Raisuddin, Sheikh
Source:
Toxicology 2019 v.428 pp. 152299
ISSN:
0300-483X
Subject:
bisphenol A, blood-brain barrier, body weight, cerebral cortex, cytokines, endocrine-disrupting chemicals, enzymes, genes, humans, males, memory disorders, messenger RNA, mice, muscles, myelin basic protein, myelin sheath, neurons, neurotoxicity, oligodendroglia, oxidative stress, protein synthesis, sclerosis, structural proteins
Abstract:
Bisphenol A (BPA) is a ubiquitously distributed endocrine disrupting chemical (EDC). BPA exposure in humans has been a matter of concern due to its increased application in the products of day to day use. BPA has been reported to cause toxicity in almost all the vital organ systems even at a very low dose levels. It crosses the blood brain barrier and causes neurotoxicity. We studied the effect of BPA on the cerebral cortex of C57BL/6J mice and examined whether BPA exposure alters the expression of axonal and myelin structural proteins. Male mice were dosed orally to 40 μg and 400 μg BPA/kg body weight for 60 days. BPA exposure resulted in memory loss, muscle coordination deficits and allodynia. BPA exposure also caused degeneration of immature and mature oligodendrocytes as evaluated by decreased mRNA levels of 2′,3′-cyclic nucleotide 3′ phosphodiesterase (CNPase), nestin, myelin basic protein (MBP) and myelin-associated glycoprotein-1 (MAG-1) genes revealing myelin related pathology. It was observed that subchronic BPA exposure caused neuroinflammation through deregulation of inflammatory cytokines mRNA and protein expression which further resulted into neurotoxicity through axonal as well as myelin degeneration in the brain. BPA also caused increased oxidative stress in the brain. Our study indicates long-term subchronic low dose exposure to BPA has the potential to cause axonal degeneration and demyelination in the oligodendrocytes and neurons which may have implications in neurological and neuropsychological disorders including multiple sclerosis (MS), neuromyelitis optica and others.
Agid:
6717031