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Electrochemical sensor using gold nanoparticles and plasma pretreated graphene based on the complexes of calcium and Troponin C to detect Ca2+ in meat

Author:
Fan, Xiaoquan, Xing, Lujuan, Ge, Panwei, Cong, Laixin, Hou, Qin, Ge, Qingfeng, Liu, Rui, Zhang, Wangang, Zhou, Guanghong
Source:
Food chemistry 2019
ISSN:
0308-8146
Subject:
bovine serum albumin, calcium, chemical composition, detection limit, electrochemistry, graphene, mixing, nanogold, oxygen, pork, sensors (equipment), troponin C
Abstract:
A simple electrochemical sensor was developed to determine the concentration of Ca2+ in meat. Graphene was treated with oxygen plasma for 10 s and 30 s comparing with the pristine graphene. Through analyzing morphology and chemical composition, the graphene with the lowest defect density was chosen to mix with bovine serum albumin molecule-functionalized gold nanoparticles. It was interesting that only a few gold nanoparticles were trapped in the graphene with 10 s plasma treatment. Then, under the optimal condition measured, the limit of detection was detected as 3.9 × 10-8 M with a linear relationship from 5 × 10-8 to 3 × 10-4 M. Finally, the proposed electrochemical method was applied to detect Ca2+ in the pork sample with stability and reproducibility verified by parallel detections. Thus, the proposed method demonstrates its potential for effectively detecting Ca2+ in meat and prominently reduces time consumption on operations and pretreatments.
Agid:
6718025