Main content area

Electrochemical sensor using gold nanoparticles and plasma pretreated graphene based on the complexes of calcium and Troponin C to detect Ca2+ in meat

Fan, Xiaoquan, Xing, Lujuan, Ge, Panwei, Cong, Laixin, Hou, Qin, Ge, Qingfeng, Liu, Rui, Zhang, Wangang, Zhou, Guanghong
Food chemistry 2019
bovine serum albumin, calcium, chemical composition, detection limit, electrochemistry, graphene, mixing, nanogold, oxygen, pork, sensors (equipment), troponin C
A simple electrochemical sensor was developed to determine the concentration of Ca2+ in meat. Graphene was treated with oxygen plasma for 10 s and 30 s comparing with the pristine graphene. Through analyzing morphology and chemical composition, the graphene with the lowest defect density was chosen to mix with bovine serum albumin molecule-functionalized gold nanoparticles. It was interesting that only a few gold nanoparticles were trapped in the graphene with 10 s plasma treatment. Then, under the optimal condition measured, the limit of detection was detected as 3.9 × 10-8 M with a linear relationship from 5 × 10-8 to 3 × 10-4 M. Finally, the proposed electrochemical method was applied to detect Ca2+ in the pork sample with stability and reproducibility verified by parallel detections. Thus, the proposed method demonstrates its potential for effectively detecting Ca2+ in meat and prominently reduces time consumption on operations and pretreatments.