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Sodium butyrate alleviates lipopolysaccharide-induced endometritis in mice through inhibiting inflammatory response

Guo, Jian, Wang, Yinan, Jiang, Peng, Yao, Hongmei, Zhao, Caijun, Hu, Xiaoyu, Cao, Yongguo, Zhang, Naisheng, Fu, Yunhe, Shen, Haiqing
Microbial pathogenesis 2019 v.137 pp. 103792
Western blotting, anaerobes, anti-inflammatory activity, bacteria, calving, dairy cows, digestive system, endometritis, enzyme-linked immunosorbent assay, eosin, fermentation, histone deacetylase, inflammation, interleukin-1beta, intraperitoneal injection, lipopolysaccharides, mice, myeloperoxidase, necrosis, neoplasms, neutrophils, protective effect, short chain fatty acids, signal transduction, sodium butyrate, therapeutics, tissues, transcription factor NF-kappa B, tumor necrosis factor-alpha, uterus
Endometritis is commonly occurred in dairy cows after calving and results in a great deal of property damage. Although numerous studies have been performed to find the therapeutic agents for endometritis, the incidence of this disease remains high. Short-chain fatty acids (SCFAs), the major metabolic products of anaerobic bacteria fermentation in the gut, have been reported to exhibit anti-inflammatory properties. Therefore, the purpose of this study was to investigate the protective effects and mechanisms of sodium butyrate (SB) on lipopolysaccharide (LPS)-induced endometritis in mice. The mice were administered by intraperitoneal injection of SB at 1 h before LPS injection. 24 h later, the uterus tissues were collected. Hematoxylin and eosin (H & E) stained sections of uterus were used to determine the degree of the damage. Uterine myeloperoxidase (MPO) activity was used to analyze neutrophil granulocytes concentration. The levels of pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were measured by ELISA. The activation of the NF-κB signaling pathway proteins were detected by Western blot analysis. The results showed that SB significantly attenuated the pathological injury of the uterus tissues. SB also suppressed LPS-induced MPO activity and the production of inflammatory cytokines TNF-α and IL-1β. Furthermore, Western blot analysis showed that SB inhibited the activation of NF-κB signaling pathway. In addition, SB could inhibit histone deacetylases. In summary, SB protects against LPS-induced endometritis through HDAC inhibition.