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Co-cultivation of the anaerobic fungus Anaeromyces robustus with Methanobacterium bryantii enhances transcription of carbohydrate active enzymes

Author:
Swift, Candice L., Brown, Jennifer L., Seppälä, Susanna, O’Malley, Michelle A.
Source:
Journal of industrial microbiology & biotechnology 2019 v.46 no.9-10 pp. 1427-1433
ISSN:
1367-5435
Subject:
Methanobacterium bryantii, anaerobes, biochemical pathways, biomass, carbohydrate binding, coculture, enzymes, fungi, gene expression regulation, genes, lignocellulose, methanogens, proteins, rumen, sequence analysis, transcription (genetics)
Abstract:
Anaerobic gut fungi are biomass degraders that form syntrophic associations with other microbes in their native rumen environment. Here, RNA-Seq was used to track and quantify carbohydrate active enzyme (CAZyme) transcription in a synthetic consortium composed of the anaerobic fungus Anaeromyces robustus with methanogen Methanobacterium bryantii. Approximately 5% of total A. robustus genes were differentially regulated in co-culture with M. bryantii relative to cultivation of A. robustus alone. We found that 105 CAZymes (12% of the total predicted CAZymes of A. robustus) were upregulated while 29 were downregulated. Upregulated genes encode putative proteins with a wide array of cellulolytic, xylanolytic, and carbohydrate transport activities; 75% were fused to fungal dockerin domains, associated with a carbohydrate binding module, or both. Collectively, this analysis suggests that co-culture of A. robustus with M. bryantii remodels the transcriptional landscape of CAZymes and associated metabolic pathways in the fungus to aid in lignocellulose breakdown.
Agid:
6724144