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Behaviour of antioxidant defense system in the adaptive response to salt stress in Helianthus annuus L. cells

Davenport, S.B., Gallego, S.M., Benavides, M.P., Tomaro, M.L.
Plant growth regulation 2003 v.40 no.1 pp. 81-88
Helianthus annuus, in vitro culture, callus, salt stress, salt tolerance, plant adaptation, antioxidant activity, lipid peroxidation, culture media, sodium chloride, dose response, cell growth, plant proteins, oxidation, chemical constituents of plants, glutathione, quantitative analysis, glutathione reductase (NADPH), glutathione dehydrogenase (ascorbate), catalase, enzyme activity, cell lines, genetic variation
A relationship between the antioxidant defense system and salt tolerance in two types of sunflower calli differing in salt sensitivity was studied. No reduction in growth occurred in the NaCl-salt-adapted cell line (T) when grown on 175 mM NaCl but growth of the salt-stressed cell line (S) was reduced by 83%. Lipid peroxidation and protein oxidation increased during acute stress of salt stressed cells at 14 and 28 d of the experiment, while salt-adapted calli (T) remained similar to non-shocked (C) values. The antioxidant defense system of callus adapted to growth under NaCl responded differently to 175 mM of salt compared with the corresponding controls under shock treatment. Salt-adapted and salt-stressed calli showed a similar pattern in GSH content at day 14 but at day 28 in S calli, GSH content was increased 100% over the non-shocked calli, while T calli returned to the initial values. In the salt-stressed calli, a general decrease in all the antioxidant enzymes studied (except for glutathione reductase and dehydroascorbate reductase activities) was observed at day 28. Except for catalase, the antioxidant enzymes were elevated constitutively in adapted calli as compared to stressed cells, when both were grown in the absence of NaCl (time 0), and remained unaltered until 28 d after the beginning of the experiment. These results suggest the involvement of an enzymatic antioxidant defense system in the adaptive response to salt stress in Helianthus annuus L. cells.