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Extracellular matrix substrata alter adipocyte yield and lipogenesis in primary cultures of stromal-vascular cells from human adipose

Author:
O'Connor, K.C., Song, H., Rosenzweig, N., Jansen, D.A.
Source:
Biotechnology letters 2003 v.25 no.23 pp. 1967-1972
ISSN:
0141-5492
Subject:
adipose tissue, adipocytes, humans, cultured cells, lipogenesis
Abstract:
The stromal-vascular fraction of human adipose was subjected to in vitro adipogenesis on different extracellular matrix substrata. Adipose tissue was harvested from the breast of 25 to 45 year-old female patients undergoing elective surgery. After 24 d, less than 5% of stromal-vascular cells had converted to adipocytes on fibronectin, 13% to 28% on tissue culture plastic and collagen I; and 59% ± 7% on Matrigel. Lipid volume surpassed 4.5 x 10³ micrometer³ cell⁻¹ for Matrigel and was 30% lower for the other substrata. Cell proliferation was evident for Matrigel and fibronectin, and cell spreading was most pronounced for fibronectin with a projected area exceeding 3 x 10³ micrometer² cell⁻¹. These results are relevant to the design of an adipose implant, providing insight into its feasibility and scaffold composition.
Agid:
672803