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Identification of Polyunsaturated Triacylglycerols and C=C Location Isomers in Sacha Inchi Oil by Photochemical Reaction Mass Spectrometry Combined with Nuclear Magnetic Resonance Spectroscopy

Li, Pan, Deng, Jiewei, Xiao, Nan, Cai, Xin, Wu, Qiaojin, Lu, Zhi, Yang, Yunyun, Du, Bing
Food chemistry 2019
Plukenetia volubilis, benzophenones, food chemistry, isomers, mass spectrometry, nuclear magnetic resonance spectroscopy, nutritive value, oils, photochemical reactions, seeds, solvents, triacylglycerols
Sacha inchi oil is derived from the seeds of Plukenetia volubilis L. and has great nutritional value due to its high contents of active polyunsaturated triacylglycerols (PUTAGs). In this study, we developed a methodology combined Paternò-Büchi reaction nanoelectrospray ionization mass spectrometry (PB-nanoESI-MS) and nuclear magnetic resonance (NMR) to identify C=C locations and isomers of PUTAGs in sacha inchi oil. Benzophenone was used as the PB reagent, and the optimized solvent composition (methanol:chloroform=9:1) allowed for PUTAGs and their PB products to be detected with higher intensities. In addition, we made efforts to interpret the MS2 spectra for identification lipid species. A series of C57-PUTAGs and C59-PUTAGs were detected and identified via high-resolution PB-nanoESI-MS, and the predominant PUTAGs were TAG 18:1(Δ9)_18:3(Δ9,12,15)_18:3(Δ9,12,15) and TAG 18:2(Δ9,12)_18:2(Δ9,12)_18:3 (Δ9,12,15), which demonstrated that the PB-nanoESI-MS approach in this study provides help in promoting the development of structural determination of triacylglycerols in food chemistry.