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Application of high-pressure treatment improves the in vitro protein digestibility of gel-based meat product

Xue, Siwen, Wang, Chong, Kim, Yuan H. Brad, Bian, Guanglian, Han, Minyi, Xu, Xinglian, Zhou, Guanghong
Food chemistry 2020 v.306 pp. 125602
batters, digesta, digestible protein, high pressure treatment, in vitro digestibility, intestines, myofibrillar proteins, peptides, proteins, rabbit meat, spectrometers
Effects of high-pressure treatments (HPT, 100–300 MPa, 9 min, 25 °C) on the in vitro digestibility of gel-type meat products were studied using a simulated digestion-model. In vitro digestibilities of the cooked rabbit meat batters throughout the simulated oral-, gastric-, and intestinal-phases were determined. Peptides in the intestinal digesta were identified via Mass Spectrometer. Results revealed that in vitro digestibilities of HPT-samples were higher than the control (1.98%, 6.13% and 61.31% for oral-, gastric- and intestinal-phase respectively) throughout the digestion (P < 0.05). Alterations of the peptide profiles were induced by HPT, showing HPT-specific patterns of mutual peptides in the digestive products. Coupled with the identifications of salt-soluble proteins from raw batters, it was confirmed that myofibrillar proteins account for the major contribution to the HPT-induced changes. The results indicated that HPT can potentially be an effective technology to improve the digestibility of meat products.