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Phenotypic evaluation and characterization of 21 industrial Saccharomyces cerevisiae yeast strains

Author:
Kong, In Iok, Turner, Timothy Lee, Kim, Heejin, Kim, Soo Rin, Jin, Yong-Su
Source:
FEMS yeast research 2018 v.18 no.1
ISSN:
1567-1364
Subject:
Saccharomyces cerevisiae, anaerobic conditions, ethanol, fermentation, fuels, genetically engineered microorganisms, glucose, heat tolerance, host strains, lignocellulose, pH, phenotype, screening, sporulation, value added, yeasts
Abstract:
Microorganisms have been studied and used extensively to produce value-added fuels and chemicals. Yeasts, specifically Saccharomyces cerevisiae, receive industrial attention because of their well-known ability to ferment glucose and produce ethanol. Thousands of natural or genetically modified S. cerevisiae have been found in industrial environments for various purposes. These industrial strains are isolated from industrial fermentation sites, and they are considered as potential host strains for superior fermentation processes. In many cases, industrial yeast strains have higher thermotolerance, increased resistances towards fermentation inhibitors and increased glucose fermentation rates under anaerobic conditions when compared with laboratory yeast strains. Despite the advantages of industrial strains, they are often not well characterized. Through screening and phenotypic characterization of commercially available industrial yeast strains, industrial fermentation processes requiring specific environmental conditions may be able to select an ideal starting yeast strain to be further engineered. Here, we have characterized and compared 21 industrial S. cerevisiae strains under multiple conditions, including their tolerance to varying pH conditions, resistance to fermentation inhibitors, sporulation efficiency and ability to ferment lignocellulosic sugars. These data may be useful for the selection of a parental strain for specific biotechnological applications of engineered yeast.
Agid:
6734224