Jump to Main Content
Red lentil supplementation reduces the severity of dextran sodium sulfate-induced colitis in C57BL/6 male mice
- Graf, Daniela, Monk, Jennifer M., Wu, Wenqing, Wellings, Hannah R., Robinson, Lindsay E., Power, Krista A.
- Journal of functional foods 2020 v.64 pp. 103625
- Lens culinaris, adjuvants, binding proteins, biomarkers, blood serum, colitis, colon, cytokines, dextran, diet therapy, dietary supplements, drinking water, epithelium, histology, inflammation, lentils, lipopolysaccharides, males, mice, nutrition risk assessment, occludins, patients, phenolic compounds, phenotype, signs and symptoms (animals and humans), sodium, sodium sulfate
- Lentils (Lens culinaris L.) are a protein-rich plant food, also enriched in fibre and phenolic compounds that may reduce intestinal-associated disease risk. Male C57Bl/6 mice were pre-fed a basal diet (BD) or isocaloric 20% red lentil-supplemented diet (LD) for 3 weeks and acute colitis was induced via dextran sodium sulfate (DSS, 2% w/v in drinking water) for 5 days. LD-fed mice exhibited reduced (i) clinical symptoms, (ii) colon histological damage, and (iii) colonic pro-inflammatory cytokine levels. Additionally, biomarkers of improved colon epithelial barrier integrity and mucosal repair mediators were increased in LD mice (e.g. colonic IL-22, Relmβ, and occludin expression, and serum lipopolysaccharide binding protein). Collectively, the severity of the DSS-induced acute colitis phenotype in mice was attenuated by red lentil dietary supplementation, indicating that lentils may serve as a potential adjuvant dietary therapy in patients with colitis-associated diseases to help limit colonic inflammation and restore barrier function.