U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

Antigenic characterization of highly pathogenic avian influenza A(H5N1) viruses with chicken and ferret antisera reveals clade-dependent variation in hemagglutination inhibition profiles.

Author:
Diep Thi Nguyen, Samuel S. Shepard, David Francis Burke, Joyce Jones, Sharmi Thor, Long Van Nguyen, Tho Dang Nguyen, Amanda Balish, Dang Nguyen Hoang, Thanh Long To, Munir Iqbal, David E. Wentworth, Erica Spackman, H. Rogier Van Doorn, C. Todd Davis, Juliet E. Bryant
Source:
Emerging microbes & infections 2018 v.7 no.1 pp. 1-15
ISSN:
2222-1751
Subject:
Influenza A virus, antigenic variation, antiserum, avian influenza, chickens, disease prevention, ferrets, hemagglutination inhibition test, influenza vaccination, influenza vaccines, viral antigens, Vietnam
Abstract:
Highly pathogenic avian influenza (HPAI) A(H5N1) viruses pose a significant economic burden to the poultry industry worldwide and have pandemic potential. Poultry vaccination against HPAI A(H5N1) viruses has been an important component of HPAI control measures and has been performed in Vietnam since 2005. To systematically assess antigenic matching of current vaccines to circulating field variants, we produced a panel of chicken and ferret antisera raised against historical and contemporary Vietnamese reference viruses representing clade variants that were detected between 2001 and 2014. The antisera were used for hemagglutination inhibition (HI) assays to generate data sets for analysis by antigenic cartography, allowing for a direct comparison of results from chicken or ferret antisera. HI antigenic maps, developed with antisera from both hosts, revealed varying patterns of antigenic relationships and clustering of viruses that were dependent on the clade of viruses analyzed. Antigenic relationships between existing poultry vaccines and circulating field viruses were also aligned with in vivo protection profiles determined by previously reported vaccine challenge studies. Our results establish the feasibility and utility of HPAI A(H5N1) antigenic characterization using chicken antisera and support further experimental and modeling studies to investigate quantitative relationships between genetic variation, antigenic drift and correlates of poultry vaccine protection in vivo.
Agid:
6743197
Handle:
10113/6743197