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Study of proteins with mercury in fish from the Amazon region
- Cavecci-Mendonça, Bruna, Cavalcante de Souza Vieira, José, Monteiro de Lima, Paula, Leite, Aline Lima, Buzalaf, Marília Afonso Rabelo, Zara, Luiz Fabrício, de Magalhães Padilha, Pedro
- Food chemistry 2020 v.309 pp. 125460
- atomic absorption spectrometry, bioinformatics, electrospray ionization mass spectrometry, fish, fractionation, hydroelectric power, mercury, power plants, proteins, rivers, triose-phosphate isomerase, two-dimensional gel electrophoresis, Amazonia
- The high concentrations of mercury found in Amazon have been intensively studied by the scientific community in the last decades. These mercurial species bind preferentially to proteins. Therefore, this work proposal sought to obtain the fractionation, identification and study of mercury – bound proteins present in samples of muscular and hepatic tissue from fish collected in the reservoir of the Jirau Hydroelectric Power Plant – on the Madeira River. Two-dimensional electrophoresis (2D-PAGE) for protein fractionation, graphite furnace atomic absorption spectrometry (GFAAS) for the quantification of mercury and Mass Spectrometry (ESI-MS/MS) were used for the identification of proteins. Concluding the work with analysis of graphics from the Blast2go program. Two mercury – bound proteins were identified as triosephosphate isomerase A and Protein FAM45A. The data generated by the bioinformatics programs confirm the tendency of these proteins to be linked to mercury and elucity the possibles existing physiological and cellular interactions.