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Oral immunization using alginate microparticles as a useful strategy for booster vaccination against fish lactoccocosis
- Romalde, J.L., Luzardo-Alvarez, A., Ravelo, C., Torano, A.E., Blanco-Mendez, J.
- Aquaculture 2004 v.236 no.1-4 pp. 119-129
- Lactococcus garvieae, animal pathogenic bacteria, Oncorhynchus mykiss, trout, anadromous fish, fish culture, fish diseases, bacterial infections, disease prevention, oral vaccination, alginates, encapsulation
- In this work, oral vaccination with encapsulated and non-encapsulated antigens was preliminary evaluated as alternative immunization procedures against trout lacotococcosis. Fish were oral immunized with a variety of different Lactococcus garvieae vaccines including encapsulated and non-encapsulated bacterial cells. An aqueous-based bacterin administered by intraperitoneal injection (i.p.) was employed as positive control. The best protective rates by oral immunization were obtained with the alginate-encapsulated vaccine (RPS of 50%); this, however, does not warrant the use of this formulation as primary immunization method. We also evaluated the efficacy of this alginate-encapsulated vaccine as booster immunization strategy. Fish were primary i.p. vaccinated with the aqueous-based vaccine and 3 months later were boostered with the oral vaccine. Four weeks after revaccination protection reached RPS values of 87%, which indicated the value of this encapsulated vaccine to increase the duration of the protection of rainbow trout against lacotoccosis.