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Autoluminescence-Free Prostate-Specific Antigen Detection by Persistent Luminous Nanorods and Au@Ag@SiO₂ Nanoparticles
- Zhao, Yuan, Zheng, Fangjie, Shi, Lixia, Liu, Han, Ke, Wei
- ACS applied materials & interfaces 2019 v.11 no.43 pp. 40669-40676
- antigen detection, aptasensors, bioassays, biomarkers, color, detection limit, fluorescent antibody technique, gold, light intensity, lighting, luminescence, nanoparticles, nanorods, neoplasms, optical properties, prostate-specific antigen, screening, silica, silver
- The sensitive detection of biomarker with high selectivity and accuracy is of remarkable significance for the early screening and clinical diagnosis of malignant cancer. Persistent phosphors play a role in illumination-free biosensing owing to their unparalleled optical properties, which produces long-lasting luminescence without the illumination to eliminate the interference of autofluorescence and scattering light from biological matrix. In this study, ZnGeO:Mo persistent luminescence nanorods (PLNRs) were prepared and showed tunable luminescence intensity and decay patterns. An excitation-free luminescent aptasensor was proposed for the detection of prostate specific antigen (PSA) by using ZnGeO:Mo PLNRs as probes and Au@Ag@SiO₂ nanoparticles as quenchers. Under optimal conditions, the limit of detection for PSA was 9.2 pg mL–¹. This work creates a possibility for the preparation of multiplex colored persistent luminescence nanomaterials with desired decay patterns for multiple bioassay and time-resolved fluoroimmunoassay.