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Clitoria ternatea L. petal bioactive compounds display antioxidant, antihemolytic and antihypertensive effects, inhibit α-amylase and α-glucosidase activities and reduce human LDL cholesterol and DNA induced oxidation

Author:
Escher, Graziela Bragueto, Marques, Mariza Boscacci, do Carmo, Mariana Araújo Vieira, Azevedo, Luciana, Furtado, Marianna Miranda, Sant'Ana, Anderson S., da Silva, Marcia Cristina, Genovese, Maria Inês, Wen, Mingchun, Zhang, Liang, Oh, Won Young, Shahidi, Fereidoon, Rosso, Neiva Deliberali, Granato, Daniel
Source:
Food research international 2020 v.128 pp. 108763
ISSN:
0963-9969
Subject:
Clitoria ternatea, DNA, absorption, alpha-amylase, alpha-glucosidase, antihypertensive effect, antimicrobial properties, antioxidant activity, bioactive compounds, cell lines, cleavage (chemistry), corolla, cytotoxicity, enzyme activity, enzyme inhibition, erythrocytes, freeze drying, functional properties, hemolysis, humans, inhibitory concentration 50, lethal concentration 50, lipid peroxidation, low density lipoprotein cholesterol, oxidation, phenolic compounds, principal component analysis, protective effect, reactive oxygen species, temperature
Abstract:
The purpose of this study was to use a statistical approach to optimise the experimental conditions regarding the extraction of bioactive compounds, and to analyse the in vitro functional properties of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of Clitoria ternatea petals. The results showed that the factors of temperature and time influenced the extraction of phenolic compounds, antioxidant activity and the physicochemical parameters. Simultaneous optimisation showed that the same levels of bioactive compounds were extracted when using temperatures from 11.7 to 68.3 °C and times from 8.47 to 51.12 min. Principal component analysis revealed the experimental conditions that provided the extraction producing the highest level of phenolic content (40 °C/30 min). The CLE showed antimicrobial activity; protective effect against hemolysis of erythrocytes; inhibition of α-amylase, α-glucosidase and angiotensin-I-converting (ACE-I) enzymes; and inhibition of lipid peroxidation. The CLE and PPE demonstrated oxygen radical absorption capacity; inhibition of DNA strand scission; inhibition of LDL cholesterol oxidation; intracellular antioxidant activity against reactive oxygen species (>100 μg/mL); and no cytotoxicity (IC₅₀, GI₅₀ and LC₅₀ > 900 μg/mL) against A549, HCT8 and IMR90 cell lines.
Agid:
6752544