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Characterization of glycerophospholipid molecular species in muscles from three species of cephalopods by direct infusion-tandem mass spectrometry

Shen, Yan, Xie, Hong-Kai, Liu, Zhong-Yuan, Lu, Ting, Yu, Zhuo-Liang, Zhang, Li-Hua, Zhou, Da-Yong, Wang, Tong
Chemistry and physics of lipids 2020 v.226 pp. 104848
Loligo, Octopodidae, cholesterol, diacylglycerols, docosahexaenoic acid, eicosapentaenoic acid, free fatty acids, healthy diet, humans, lysophosphatidylcholine, monounsaturated fatty acids, muscles, omega-3 fatty acids, phosphatidylethanolamines, phosphatidylserines, saturated fatty acids, sphingomyelins, tandem mass spectrometry, triacylglycerols
More than 200 molecular species of glycerophospholipids (GP) including glycerophosphocholine (GPC), glycerophosphoethanolamine (GPE), glycerophosphoserine (GPS), lysoglycerophosphocholine (LGPC), lysoglycerophosphoethanolamine (LGPE) and lysoglycerophosphoserine (LGPS), as well as 18 kinds of sphingomyelin (SM) were characterized by using a direct infusion-tandem mass (MS/MS) spectrometry method for lipids from the muscles of cephalopods Sepiella maindroni, Octopus ocellatus and Loligo chinensis for the first time. The majority of the GP molecular species contained long-chain omega-3 polyunsaturated fatty acids (n-3 LC-PUFA), especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Therefore, cephalopods can be a good possible source of dietary GP carrying n-3 LC-PUFA. The total lipids were composed of phospholipid (PL, 72.29–83.32 wt% of total lipids), cholesterol (12.70–23.60 wt% of total lipids), triacylglycerol (1.86–2.93 wt% of total lipids), diacylglycerol (0.15–1.09 wt% of total lipids), monoacylglycerol (0.06-0.18 wt% of total lipids) and free fatty acid (0.72–1.86 wt% of total lipids). For PL, phosphatidylcholine (44.47–62.30 mol%), phosphatidylethanolamine (22.57–39.08 mol%), phosphatidylserine (6.15–10.18 mol%), phosphatidylglycerol (0.68–3.11 mol%), phosphatidylinositol (2.41–7.15 mol%) and lysophosphatidylcholine (1.84–5.24 mol%) were detected. Furthermore, the total lipids from the muscles of cephalopods Sepiella maindroni, Octopus ocellatus and Loligo chinensis contained 41.80–50.02 mol% of saturated fatty acids, 11.53–21.54 mol% of monounsaturated fatty acids and 36.67–40.82 mol% of PUFA, whilst DHA (15.25–26.71 mol%) and EPA (6.29–16.57 mol%) were found to account for the majority of the PUFA. With these data presented, cephalopod muscle can be considered as a healthy food for humans.