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Prosystemin-antimicrobial-peptide fusion reduces tomato late blight lesion expansion
- Jones, R.W., Ospina-Giraldo, M., Clemente, T.
- Molecular breeding 2004 v.14 no.1 pp. 83-89
- Solanum lycopersicum var. lycopersicum, tomatoes, transgenic plants, Agrobacterium tumefaciens, genetic transformation, gene transfer, Solanum tuberosum, potatoes, recombinant fusion proteins, polypeptides, antimicrobial peptides, plant proteins, gene expression, disease resistance, genetic resistance, Phytophthora infestans, plant pathogenic fungi, leaf blight, fungal diseases of plants, leaves
- Antimicrobial peptides offer a new method for controlling pathogens, however, many promising peptides are too small for direct production in plants. A protein delivery system was developed based on a proteolytic mechanism used by Solanaceous plants to produce the very small (18 amino acid) signaling peptide systemin from the polypeptide prosystemin. Fusion of the gene encoding the 23 kDa protein prosystemin with the antimicrobial peptide (pep11) sequence, replacing the systemin sequence, allowed for expression in transgenic tomato plants. Six days after inoculation with the late blight pathogen Phytophthora infestans, detached leaflets of transgenic tomato (Rutgers) exhibited a reduction in lesion size of at least 50 percent.