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Remediation mechanism of endophytic fungus Phomopsis liquidambaris on phenanthrene in vivo

Fu, Wan-Qiu, Xu, Man, Sun, Kai, Chen, Xiang-Liang, Dai, Chuan-Chao, Jia, Yong
Chemosphere 2020 v.243 pp. 125305
Diaporthe liquidambaris, Oryza sativa, biodegradability, bioremediation, chlorophyll, endophytes, energy, enzyme activity, food safety, fungi, gene expression, genes, human health, phenanthrenes, rice, roots, seedlings, soil, superoxide dismutase, symbiosis, viability
Phenanthrene can easily be absorbed into the plant from the soil and cannot be effectively degraded in it. Thus, it is greatly hazardous for food safety and human health. In our study, the biodegradability and remediation mechanism of endophytic fungus Phomopsis liquidambaris on phenanthrene in vivo of rice (Oryza sativa L.) was detected. The results showed that the fungus could successfully establish a symbiotic relationship with rice, thus had the potential to degrade phenanthrene absorbed into the plant. Changes of phenanthrene-degrading genes of fungus in the combined system were consistent with the trends of their corresponding enzymatic activities, and the phenanthrene-degrading enzyme activities and gene expression levels in roots of rice were higher than those in the shoot. Moreover, the combined system can enhance bioremediation by increasing root viability, chlorophyll content, and energy supply. The combined system had also significantly increased the PPO activity and SOD activity in shoot compared with the control treatment, while decreased the content of MDA when remediation in vivo. The study on the degradation mechanism of the combined system will help us to increase the practical application potential of endophyte to effectively repair contamination absorbed into plant seedlings.