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Microbial production of O-methylated flavanones from methylated phenylpropanoic acids in engineered Escherichia coli
- Cui, Heqing, Song, Myoung Chong, Lee, Ji Young, Yoon, Yeo Joon
- Journal of industrial microbiology & biotechnology 2019 v.46 no.12 pp. 1707-1713
- Escherichia coli, Hordeum vulgare, Oryza sativa, bioactive properties, biochemical pathways, ferulic acid, hesperetin, ligases, methylation, naringenin-chalcone synthase
- Methylated flavonoids possess improved bioactivities compared to their unmethylated counterparts. In this study, for the efficient production of O-methylated flavonoids from simple methylated phenylpropanoic acids, a recombinant Escherichia coli strain expressing 4-coumarate:coenzyme A ligase (4CL) from Oryza sativa and chalcone synthase (CHS) from Hordeum vulgare was constructed; this strain produced significant amount of homoeriodictyol (~ 52 mg/L) as well as a few amount of hesperetin (0.4 mg/L), respectively, from ferulic acid and 4-methylcaffeic acid. This demonstrates, for the first time, that the scarce but valuable methylated flavanones can be successfully produced from methylated phenylpropanoic acids in a microbial host via an artificial biosynthetic pathway consisting of 4CL and CHS that can accept O-methylated precursors.