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Preliminary examination of factors affecting Agrobacterium tumefaciens-mediated transformation of marula, Sclerocarya birrea subsp. caffra (Anacardiaceae)

Author:
Mollel, M.H.N., Goyvaerts, E.M.A.
Source:
Plant cell, tissue, and organ culture 2004 v.79 no.3 pp. 321-328
ISSN:
0167-6857
Subject:
indigenous species, Sclerocarya birrea, fruit trees, genetic improvement, genetic transformation, Agrobacterium tumefaciens, gene transfer, transgenic plants, gene expression, shoots, in vitro culture, in vitro regeneration, culture media, benzyladenine, gibberellins, kinetin, dose response, micropropagation, methodology, Africa
Abstract:
In planta Agrobacterium tumefaciens-mediated transformation of marula, Sclerocarya birreasubsp. caffra(Anacardiaceae) resulted in chimeric transgenic in vitro shoots at a rate of 0.8--1.5%. Average transgene expression rates of 33.1 and 1.3% GUS-positive explants were observed on days 3 and 6 after agroinfection, respectively. One to 4 GUS-positive zones were observed per GUS-positive explant section. Addition of acetosyringone (100 micromolar) during co-cultivation significantly improved transient transformation efficiency as determined by the percentage of GUS-positive explants on day 3 (p< 0.05) whereas wounding did not show a significant effect. However, wounding and acetosyringone acted antagonistically reducing transient transformation rates.
Agid:
676451