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Aptamers in RNA-based switches of gene expression
- Spöring, Maike, Finke, Monika, Hartig, Jörg S
- Current opinion in biotechnology 2020 v.63 pp. 34-40
- eukaryotic cells, gene expression, gene therapy, immunogenicity, oligonucleotides, regulatory proteins
- The ability to control gene expression via small molecule effectors is important in basic research as well as in future gene therapy applications. Although transcription factor-based systems are widely used, they are not well suited for certain applications due to a lack of functionality, limited available coding space, and potential immunogenicity of the regulatory proteins. RNA-based switches fill this gap since they can be designed to respond to effector compounds utilizing ligand-sensing aptamers. These systems are very modular since the aptamer can be combined with a variety of different expression platforms. RNA-based switches have been constructed that allow for controlling gene expression in diverse contexts. Here we discuss latest developments and applications of aptamer-based gene expression switches in eukaryotes.