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A novel gold nanostars-based fluorescent aptasensor for aflatoxin B1 detection
- Wei, Min, Zhao, Fei, Xie, Yanli
- Talanta 2020 v.209 pp. 120599
- aflatoxin B1, aptasensors, complementary DNA, corn flour, fluorescence, nanogold, oligonucleotides
- In this work, a simple and sensitive fluorescent aptasensor for aflatoxin B1 (AFB₁) detection was proposed using gold nanostars (AuNSs) as a novel fluorescence quenching material. Carboxyfluorescein-labeled complementary DNA with hairpin structure (FAM-labeled HP) was designed to hybridize with AFB₁ aptamer to form double-stranded DNA, resulting in the opening of hairpin structure. When double-stranded DNA was modified on AuNSs surface, FAM was far from AuNSs and produced a strong fluorescence intensity. The introduction of AFB₁ in the system led to the specific interaction of AFB₁ and aptamer, and changed the conformation of aptamer, inducing the release of aptamer from double-stranded DNA and the restoration of hairpin structure. Fluorescence quenching occurred when FAM was close to AuNSs, and the fluorescence intensity decreased. In the presence of 5 ng/mL AFB₁, ΔF/F₀ of the AuNSs/FAM-labeled HP/Apt was ~44.2%, higher than that of the AuNPs/FAM-labeled HP/Apt, indicating the better quenching effect of AuNSs. The change of fluorescence intensity linearly increased by adding AFB₁ in the concentration range of 0.1 ng/mL-10 ng/mL, with the LOD of 21.3 pg/mL. The proposed aptasensor exhibited good selectivity in the presence of other toxins at 10-fold concentration of AFB₁, and showed satisfactory recovery in the range of 92%–112% toward AFB₁ detection in spiked corn flour sample.