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Characterization of porcine reproductive and respiratory syndrome virus (ORF5 RFLP 1-7-4 viruses) in northern China
- Xie, Changzhan, Ha, Zhuo, Nan, Fulong, Zhang, Ying, Zhang, Han, Li, Jinfeng, Zhang, Ping, Han, Jicheng, Zhang, He, Zhuang, Xinyu, Zhang, Jinyong, Lu, Huijun, Jin, Ningyi
- Microbial pathogenesis 2020 v.140 pp. 103941
- Porcine reproductive and respiratory syndrome virus, disease outbreaks, etiological agents, genome, genotype, herds, lungs, open reading frames, piglets, porcine reproductive and respiratory syndrome, restriction fragment length polymorphism, viruses, weaning, China
- The porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of porcine reproductive and respiratory syndrome (PRRS). Disease outbreaks caused by NADC30-like PRRSV strains were a bit prevalent in China in recent years. In the present study, two newly emerged PRRSV strains, which were designated as PRRSV-ZDXYL-China-2018-1 and PRRSV-ZDXYL-China-2018-2 strains were found from piglets’ lung tissues in Northern China. The virus belongs to lineage 1 of the PRRSV genotype 2 and is closely related to US strains that possess the open reading frame (ORF5) restriction fragment length polymorphism (RFLP) 1-7-4. The two strains were identified from infected weaning piglet herds in Zhaodong City, Heilongjiang province of China. The complete genome of the PRRSV-ZDXYL-China-2018-1 and PRRSV-ZDXYL-China-2018-2 strains were 15093 nt and 15110 nt, and shared 96.7%–97.0% and 97.1%–97.4% similarities with the US identified, ISU10 and NADC34 strains respectively. Then the PRRSV-ZDXYL-China-2018-1 strain was successfully isolated from the clinical sample. Our results demonstrate, that the emergence of ORF5 RFLP 1-7-4-like PRRSVs in China, could pose a significant challenge to PRRSV epidemic prevention.