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Characterization of porcine reproductive and respiratory syndrome virus (ORF5 RFLP 1-7-4 viruses) in northern China

Author:
Xie, Changzhan, Ha, Zhuo, Nan, Fulong, Zhang, Ying, Zhang, Han, Li, Jinfeng, Zhang, Ping, Han, Jicheng, Zhang, He, Zhuang, Xinyu, Zhang, Jinyong, Lu, Huijun, Jin, Ningyi
Source:
Microbial pathogenesis 2020 v.140 pp. 103941
ISSN:
0882-4010
Subject:
Porcine reproductive and respiratory syndrome virus, disease outbreaks, etiological agents, genome, genotype, herds, lungs, open reading frames, piglets, porcine reproductive and respiratory syndrome, restriction fragment length polymorphism, viruses, weaning, China
Abstract:
The porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of porcine reproductive and respiratory syndrome (PRRS). Disease outbreaks caused by NADC30-like PRRSV strains were a bit prevalent in China in recent years. In the present study, two newly emerged PRRSV strains, which were designated as PRRSV-ZDXYL-China-2018-1 and PRRSV-ZDXYL-China-2018-2 strains were found from piglets’ lung tissues in Northern China. The virus belongs to lineage 1 of the PRRSV genotype 2 and is closely related to US strains that possess the open reading frame (ORF5) restriction fragment length polymorphism (RFLP) 1-7-4. The two strains were identified from infected weaning piglet herds in Zhaodong City, Heilongjiang province of China. The complete genome of the PRRSV-ZDXYL-China-2018-1 and PRRSV-ZDXYL-China-2018-2 strains were 15093 nt and 15110 nt, and shared 96.7%–97.0% and 97.1%–97.4% similarities with the US identified, ISU10 and NADC34 strains respectively. Then the PRRSV-ZDXYL-China-2018-1 strain was successfully isolated from the clinical sample. Our results demonstrate, that the emergence of ORF5 RFLP 1-7-4-like PRRSVs in China, could pose a significant challenge to PRRSV epidemic prevention.
Agid:
6791039