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Two dimensional chromatography mass spectrometry: Quantitation of chiral shifts in metabolism of propranolol in bioanalysis

Author:
Harps, Lukas C., Schipperges, Sonja, Bredendiek, Felix, Wuest, Bernhard, Borowiak, Andreas, Parr, Maria Kristina
Source:
Journal of chromatography 2020 v.1617 pp. 460828
ISSN:
0021-9673
Subject:
calibration, chromatography, excretion, humans, hydroxylation, mass spectrometry, metabolism, metabolites, oral administration, propranolol, spectrometers, urine
Abstract:
In this study a heart-cutting 2D-LC method was successfully developed and optimized in order to discriminate and quantitate (S)-propranolol, (R)-propranolol, and its hydroxy metabolites, namely the isomeric (S)-4′‑hydroxy propranolol, (R)-4′‑hydroxy propranolol, (S)-5′‑hydroxy propranolol, (R)-5′‑hydroxy propranolol, (S)-7′-hydroxy propranolol, and (R)-7′‑hydroxy propranolol in one chromatographic run. Thereby, experiments investigating chiral discrimination in ring hydroxylation of propranolol were made feasible.Analysis of human urine samples after administration of a single oral dose of 40 mg of propranolol clearly revealed considerable chiral shifts in propranolol and its 4′-, 5′-, and 7′-hydroxy metabolites. Furthermore, the excretion rates of the individual (S)- and (R)-enantiomers were continuously monitored over 24 h post administration.Studies were performed utilizing a 2D-LC system hyphenated to a triple quadrupole mass spectrometer. The chromatographic system was endued with a reversed phase column (phenyl-hexyl) in first dimension and a teicoplanin based chiral column in second dimension. The method was basically validated and successfully evaluated as robust. Calibration was performed achieving accuracy between 80% and 120%. Maximal excretion rates of (S)-propranolol, (R)-propranolol, (S)-4′‑hydroxy propranolol, (R)-4′‑hydroxy propranolol, (S)-5′‑hydroxy propranolol, (R)-5′‑hydroxy propranolol, and (R)-7′‑hydroxy propranolol were 237 ng/min, 281 ng/min, 4 ng/min, 4 ng/min, 1 ng/min, 9 ng/min, and 3 ng/min, respectively.
Agid:
6795590