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Effect of peptidoglycan amidase MSMEG_6281 on fatty acid metabolism in Mycobacterium smegmatis

Author:
Miao, Jiatong, Liu, Hanrui, Qu, Yushan, Fu, Weizhe, Qi, Kangwei, Zang, Shizhu, He, Jiajia, Zhao, Shijia, Chen, Shixing, Jiang, Tao
Source:
Microbial pathogenesis 2020 v.140 pp. 103939
ISSN:
0882-4010
Subject:
ABC transporters, Mycobacterium smegmatis, acyl coenzyme A, acyl-CoA dehydrogenase, amidase, biofilm, cell walls, fatty acid metabolism, gene targeting, liquid chromatography, mycolic acids, pathogenicity, peptides, peptidoglycans, phenotype, protein-protein interactions, proteome, proteomics, tandem mass spectrometry
Abstract:
Mycobacterium smegmatis MSMEG_6281, a peptidoglycan (PG) amidase, is essential in maintaining cell wall integrity. To address the potential roles during the MSMEG_6281-mediated biological process, we compared proteomes from wild-type M.smegmatis and MSMEG_6281 gene knockout strain (M.sm-ΔM_6281) using LC-MS/MS analysis. Peptide analysis revealed that 851 proteins were differentially produced with at least 1.2-fold changes, including some proteins involved in fatty acid metabolism such as acyl-CoA synthase, acyl-CoA dehydrogenase, MCE-family proteins, ATP-binding cassette (ABC) transporters, and MmpL4. Some proteins related to fatty acid degradation were enriched through protein-protein interaction analysis. Therefore, proteomic data showed that a lack of MSMEG_6281 affected fatty acid metabolism. Mycobacteria can produce diverse lipid molecules ranging from single fatty acids to highly complex mycolic acids, and mycobacterial surface-exposed lipids may impact biofilm formation. In this study, we also assessed the effects of MSMEG_6281 on biofilm phenotype using semi-quantitative and morphology analysis methods. These results found that M.sm-ΔM_6281 exhibited a delayed biofilm phenotype compared to that of the wild-type M.smegmatis, and the changes were recovered when PG amidase was rescued in a ΔM_6281::Rv3717 strain. Our results demonstrated that MSMEG_6281 impacts fatty acid metabolism and further interferes with biofilm formation. These results provide a clue to study the effects of PG amidase on mycobacterial pathogenicity.
Agid:
6796972