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Dynamic proteome responses to sequential reduction of Cr(VI) and adsorption of Pb(II) by Pannonibacter phragmitetus BB

Liao, Qi, Tang, Jiaqi, Wang, Haiying, Yang, Weichun, He, Lixu, Wang, Yangyang, Yang, Zhihui
Journal of hazardous materials 2020 v.386 pp. 121988
adsorption, bacterial proteins, chromium, lead, lipoproteins, nutrient transport, phosphates, physiological response, protein-protein interactions, proteome, proteomics, siderophores, sodium-hydrogen antiporter, superoxide dismutase, toxicity
Here, the microbial responses to Cr(VI) and Pb(II) with bio-removal of the metals in water by Pannonibacter phragmitetus BB were explored. The comparative bacterial proteomics showed that the intracellular and extracellular Cr(VI) reduction proteins, Pb(II) adsorption by the lipoprotein and sugar-related bacterial proteins, as well as Pb(II) precipitation by phosphate and OH⁻ were vital to the bio-removal of Cr(VI) and Pb(II). Moreover, the influx and efflux channels of Cr(VI) and Cr(III), Pb(II) transporters, extracellular siderophores for Pb(II) complexation and antioxidant proteins enabled the strain BB to resist the toxicity of Cr(VI) and Pb(II). In addition, the dynamic expression levels of the proteins related to reduction and transportation of Cr(VI), and adsorption, transportation and complexation of Pb(II) were dependent on the corresponding metal, respectively. The anti-oxidative stress system, such as superoxide dismutase, and Na⁺/H⁺ antiporters played central roles in the protein–protein interaction network to resist and detoxify Cr(VI) and Pb(II). The results of our study provide a novel insight for the physiological responses of the strain BB to the combined stresses of Pb(II) and Cr(VI).