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TILLING by Sequencing: A Successful approach to identify rare alleles in soybean populations
- Thapa Rima, Colon Militza, Rainey Katy M., Hudson Karen A.
- Genes 2019 v.10 no.12 pp. 1-9
- alleles, genetic variation, germplasm, livestock feeds, monogastric livestock, mutants, mutation, plant breeding, raffinose, reverse genetics, seeds, soybeans, stachyose, sucrose
- Soybean seeds produce valuable protein that is a major component of livestock feed. However, soybean seeds also contain the anti-nutritional raffinose family oligosaccharides (RFOs) raffinose and stachyose, which are not digestible by non-ruminant animals. While reducing RFOs in soybean seed has been a goal of soybean breeding, efforts are constrained by limited genetic variability in soybean germplasm. We used the reverse genetics TILLInG-by-Sequencing approach to identify a damaging polymorphism in the RAFFINOSE SYNTHASE3 gene. We demonstrate that this mutation, when combined as a double mutant with mutation in RAFFINOSE SYNTHASE2, eliminates nearly all of the RFOs in soybean seed, and results in increased levels of sucrose.