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Malic enzyme 1 genotype is associated with backfat thickness and meat quality traits in pigs

Vidal, O., Varona, L., Oliver, M.A., Noguera, J.L., Sànchez, A., Amills, M.
Animal genetics 2006 v.37 no.1 pp. 28-32
quantitative trait loci, pork, malic enzyme, haplotypes, thickness, meat quality, single nucleotide polymorphism, Bayesian theory, lipogenesis, chromosome mapping, genes, nucleotide sequences, genetic markers, backfat, swine
Malic enzyme 1 (ME1) is a part of the tricarboxylate shuttle that provides NADPH and acetyl-CoA required in fatty acid biosynthesis. The pig ME1 locus maps on the proximal end of chromosome 1, where a quantitative trait loci (QTL) affecting fat deposition has been previously described. We amplified fragments of 1457 and 1459 bp that corresponded to the complete coding region and the 3[prime]-untranslated region (UTR), respectively, of the pig ME1 gene. The sequences of these two fragments in pigs from three breeds (Landrace, Large White and Piétrain) contained five single nucleotide polymorphisms (SNP) in the 3[prime]-UTR: C1706T, G1762T, A1807C, C1857A and T1880A. Three haplotypes were found in two generations of a selected Landrace population: H1 (C₁₇₀₆ G₁₇₆₂ A₁₈₀₇ C₁₈₅₇ A₁₈₈₀), H2 (C₁₇₀₆ G₁₇₆₂ A₁₈₀₇ C₁₈₅₇ T₁₈₈₀) and H3 (T₁₇₀₆ T₁₇₆₂ C₁₈₀₇ A₁₈₅₇ T₁₈₈₀). Using Bayesian association analyses, significant associations (highest posterior density at 95%) between ME1 genotype and backfat (BF) thickness at 171 days and muscular pH were found in a Landrace population.