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Ameliorate effects of soybean soluble polysaccharide on adenine-induced chronic renal failure in mice

Hou, Guohua, Jin, Mingzhi, Ye, Ziyang, Zhang, Xinmiao, Huang, Qianli, Ye, Ming
International journal of biological macromolecules 2020 v.149 pp. 158-164
actin, adenine, albumins, animal models, blood, collagen, creatinine, gene expression, histopathology, kidneys, messenger RNA, mice, polysaccharides, protein content, renal failure, renal function, smooth muscle, soybeans, transforming growth factor beta, urea nitrogen
In the present study, the kidney protection effects of soluble soybean polysaccharide (SSPS) were evaluated. To address the issues, a mice model of Chronic renal failure (CRF) was established by gavage 0.2% (w/w) adenine for 3 weeks. In vivo results showed that SSPS could change the concentrations of blood urea nitrogen (BUN), creatinine (CRE), total protein (TP) and albumin (ALB), thereby affecting kidney function. In addition, Masson histopathology analysis indicated that SSPS could decrease the area of collagen fiber in the kidney tissues of CRF mice. Moreover, the results of mRNA expression and western experiment suggested that SSPS treatment could increase the expression of transforming growth factor-β (TGF-β), Smad3 and P-Smad3, while reduce the expression of α smooth muscle actin (α-SMA) when compared with the model group. These results indicated that SSPS potentially improve kidney function through TGF/Smad pathway in CRF mice.