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Effect of gga-miR-155 on cell proliferation, apoptosis and invasion of Marek’s disease virus (MDV) transformed cell line MSB1 by targeting RORA

Ding, Ke, Yu, Zu-Hua, Yu, Chuan, Jia, Yan-Yan, He, Lei, Liao, Cheng-Shui, Li, Jing, Zhang, Chun-Jie, Li, Yin-Ju, Wu, Ting-Cai, Cheng, Xiang-Chao, Zhou, Zi-Yu, Yu, Zu-Ling
BMC veterinary research 2020 v.16 no.1 pp. 23
Mardivirus, Marek disease, apoptosis, carcinogenesis, cell proliferation, chickens, gene expression regulation, interphase, microRNA, neoplasms, oncogenes, tissues
BACKGROUND: Marek’s disease (MD) is caused by the oncogenic Marek’s disease virus (MDV), and is a highly contagious avian infection with a complex underlying pathology that involves lymphoproliferative neoplasm formation. MicroRNAs (miRNAs) act as oncogenes or tumor suppressors in most cancers. The gga-miR-155 is downregulated in the MDV-infected chicken tissues or lymphocyte lines, although its exact role in tumorigenesis remains unclear. The aim of this study was to analyze the effects of gga-miR-155 on the proliferation, apoptosis and invasiveness of an MDV-transformed lymphocyte line MSB1 and elucidate the underlying mechanisms. RESULTS: The expression level of gga-miR-155 was manipulated in MSB1 cells using specific mimics and inhibitors. While overexpression of gga-miR-155 increased proliferation, decreased the proportion of G1 phase cells relative to that in S and G2 phases, reduced apoptosis rates and increased invasiveness. However, its downregulation had the opposite effects. Furthermore, gga-miR-155 directly targeted the RORA gene and downregulated its expression in the MSB1 cells. CONCLUSION: The gga-miR-155 promotes the proliferation and invasiveness of the MDV-transformed lymphocyte line MSB1 and inhibits apoptosis by targeting the RORA gene.