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Free and hidden fumonisins in Argentinean raw maize samples

Pok, P.S., Londoño, V.A. García, Aransibia, S.P., Vicente, S., Pacín, A.M., Resnik, S.L.
World mycotoxin journal 2020 v.13 no.1 pp. 109-116
alkaline hydrolysis, corn, detection limit, fluorescence, fumonisins
The concentrations of free fumonisins (FBs) and hydrolysed fumonisins (HFBs) were determined in 72 maize samples collected in 2017 from five Argentine provinces. The methodology for HFBs analysis consisted of alkaline hydrolysis followed by high performance liquid chromatography with a fluorescence detector (HPLC-FLD). The developed analytical methodology presented percentages of recovery greater than 74%. Limits of detection were 4.5, 13.0 and 12.9 μg/kg for HFB₁, HFB₂ and HFB₃, respectively. Presence of FBs was found in 86% of the samples. In all cases, the concentration of total HFBs (after the hydrolysis treatment) was superior to the free FBs content (HFBs to FBs median ratio of 2.5), which indicates the presence of hidden fumonisins in Argentinean maize. 8% of the traditionally analysed samples exceeded the limit established by the European Commission for FB₁ + FB₂. When applying alkaline hydrolysis to the samples, 24% of them exceed this limit.