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An indigenous bacterium Bacillus XZM for phosphate enhanced transformation and migration of arsenate

Author:
Wang, Jia, Xie, Zuoming, Wei, Xiaofan, Chen, Mengna, Luo, Yan, Wang, Yanxin
Source:
The Science of the total environment 2020 v.719 pp. 137183
ISSN:
0048-9697
Subject:
aquifers, arsenates, arsenic, bacteria, bacterial growth, biomass production, environmental indicators, genes, metals, phosphates, quantitative polymerase chain reaction, redox potential, sediments
Abstract:
A number of arsenate-reducing bacteria respire adsorbed As(V), producing As(III) and thus contributing to arsenic mobilization from the solid phase to the aqueous phase. Two arsenate reducing genes, arsC and arrA, were both amplified in an indigenous bacterium Bacillus XZM isolated from high arsenic aquifer sediments. The effect of phosphate input on this novel bacterium in terms of mediating the biogeochemical behavior of arsenic was investigated for the first time. The results show bacterial growth and arsenate reduction appear to increase with the addition of phosphate. Input of 1 mM phosphate reduced the negative effects of As(V) on bacterial growth, resulting in 55–60% greater biomass production compared to lower phosphate inputs (0.01 and 0.1 mM). The data of real-time quantitative PCR (qPCR) indicated arsenate was involved in the expressions of two arsenate reductase genes (arsC and arrA genes) in indigenous bacterium Bacillus XZM. Overall, the addition of phosphate (from 0.1 to 1 mM) resulted in a doubling of arsenate bio-desorption from the sediment into the aqueous medium. Oxidation-reduction potential, as an environmental indicator of the bacterial reduction of metals, declined to −200 mV in the presence of strain XZM and 1 mM phosphate in the microcosm. Phosphate input enhanced arsenic biomigration, indicating the effect of phosphate concentration should be considered when studying the biogeochemical behavior of arsenic.
Agid:
6846010