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New SNPs in the IGF2 gene and association between this gene and backfat thickness and lean meat content in Large White pigs
- Vykoukalová, Z., Knoll, A., Dvořák, J., Čepica, S.
- Journal of animal breeding and genetics 2006 v.123 no.3 pp. 204-207
- swine, mutation, quantitative trait loci, backfat, thickness, carcass composition, body composition, linkage disequilibrium, insulin-like growth factor II, genetic polymorphism, lean meat, single nucleotide polymorphism, introns, polymerase chain reaction, gene segregation, restriction fragment length polymorphism, genetic markers, genotype
- IGF2-in3-G3072A is a causative mutation for paternally expressed quantitative trait loci on the p arm of porcine chromosome 2 with substantial effect on muscle growth and backfat thickness. The linkage disequilibrium between IGF2-in3-G3072A and IGF2-in7-G162C (IGF2-NciI) in four breeds and associations between these polymorphisms and growth and meat performance in pigs of the Large White breed were analysed. A significant effect of these polymorphisms on backfat thickness and lean meat content was found. In addition, we identified two new single nucleotide polymorphisms (SNPs) in intron 7 of the gene. The existence of complete linkage disequilibrium between IGF2-in3-G3072A locus in the population under study where the locus segregated and SNPs in intron 7 of the IGF2 gene detectable with simple and reliable polymerase chain reaction-restriction fragment length polymorphism techniques (G162C, C179G and G186T) offer possibilities to use these SNPs for genotyping of quantitative trait nucleotide in Large White and Landrace breeds.