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New SNPs in the IGF2 gene and association between this gene and backfat thickness and lean meat content in Large White pigs

Vykoukalová, Z., Knoll, A., Dvořák, J., Čepica, S.
Journal of animal breeding and genetics 2006 v.123 no.3 pp. 204-207
swine, mutation, quantitative trait loci, backfat, thickness, carcass composition, body composition, linkage disequilibrium, insulin-like growth factor II, genetic polymorphism, lean meat, single nucleotide polymorphism, introns, polymerase chain reaction, gene segregation, restriction fragment length polymorphism, genetic markers, genotype
IGF2-in3-G3072A is a causative mutation for paternally expressed quantitative trait loci on the p arm of porcine chromosome 2 with substantial effect on muscle growth and backfat thickness. The linkage disequilibrium between IGF2-in3-G3072A and IGF2-in7-G162C (IGF2-NciI) in four breeds and associations between these polymorphisms and growth and meat performance in pigs of the Large White breed were analysed. A significant effect of these polymorphisms on backfat thickness and lean meat content was found. In addition, we identified two new single nucleotide polymorphisms (SNPs) in intron 7 of the gene. The existence of complete linkage disequilibrium between IGF2-in3-G3072A locus in the population under study where the locus segregated and SNPs in intron 7 of the IGF2 gene detectable with simple and reliable polymerase chain reaction-restriction fragment length polymorphism techniques (G162C, C179G and G186T) offer possibilities to use these SNPs for genotyping of quantitative trait nucleotide in Large White and Landrace breeds.