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A specific peptide with immunomodulatory activity from Pseudostellaria heterophylla and the action mechanism

Yang, Qian, Cai, Xixi, Huang, Muchen, Wang, Shaoyun
Journal of functional foods 2020 v.68 pp. 103887
Toll-like receptor 2, Western blotting, amino acid sequences, dietary supplements, endocytosis, immunomodulators, lymphocytes, molecular weight, protein hydrolysates, reversed-phase high performance liquid chromatography, secretion, signal transduction, small interfering RNA, spleen, transcription factor NF-kappa B, tumor necrosis factor-alpha
Pseudostellaria heterophylla protein hydrolysate has been reported to be a potential nutraceutical supplement to strengthen the immune system. A specific peptide from P. heterophylla protein hydrolysate with proliferation promotion effect on spleen lymphocytes was purified by RP-HPLC. The amino acid sequence of the peptide was determined to be RGPPP, with molecular weight of 522.29 Da. 100 μg/mL of RP-5 achieved the highest stimulation index of 1.27 on spleen lymphocytes and activated RAW264.7 cells resulting in up-regulating tumor necrosis factor-α (TNF-α) secretion to 827.24 pg/mL and elevating pinocytosis rate to 123.81%. Results of western-blot and siRNA experiments indicated that RP-5 activated nuclear factor-κB (NF-κB) signaling pathway through combining to TLR2 expressed on RAW264.7 cells surface. Furthermore, TNF-α secretion increased by RP-5 could be decreased to 401.68 pg/mL by pyrrolidine dithiocarbamate or BAY11-7082. These findings further the research progress of peptide with immunomodulatory activity and its action mechanism.