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Stimulatory effect of copper and zinc sulphate on plant regeneration, glutathione-S-transferase analysis and assessment of antioxidant activities in Mucuna pruriens L. (DC)

Alam, Naushad, Anis, Mohammad, Javed, Saad Bin, Alatar, Abdulrahman A.
Plant cell, tissue, and organ culture 2020 v.141 no.1 pp. 155-166
Mucuna pruriens, antioxidant activity, auxins, benzyladenine, copper, copper sulfate, explants, gardens, glutathione transferase, heavy metals, in vitro regeneration, isopentenyladenine, kinetin, legumes, roots, sandy soils, shoots, solar radiation, zinc sulfate
The effect of heavy metals, CuSO₄ and ZnSO₄ on morphogenic response of cotyledonary node explants of Mucuna pruriens (L.), a fast growing tropical medicinal legume was evaluated. The climber holds economic importance in traditional as well as modern pharmaceutical areas. Murashige and Skoog (Physiol Plant 15:473–497, 1962) medium supplemented with various strength (0.5, 2.5, 4.5, 6.5 or 8.5 µM) of BA (6-benzyladenine), Kn (Kinetin) and 2-iP (2-isopentenyl adenine) were screened to establish optimum culture conditions for in vitro regeneration using cotyledonary node explant. Optimized medium (MS + BA 2.5 µM) was augmented with different concentration of CuSO₄ (0.5–25.0 µM) and ZnSO₄ (5–150 µM) to evaluate the effect of heavy metals on regeneration potential of the explants. Among various concentrations tested, MS medium with optimized BA (2.5 µM) along with 5.0 µM CuSO₄ or 80.0 µM ZnSO₄ proved beneficial, inducing 11.20 and 13.40 shoots per explant, respectively. The results demonstrated that glutathione-S-transferase (GSTs) was involved in fighting heavy metal stress, and might also have a role in in vitro regeneration. Elongated healthy microshoots on transfer to half strength MS medium produced 3.40 roots per shoot after 28 days of incubation without the necessity of exogenous auxin. The in vitro grown regenerants were hardened in Soilrite, and then established in garden and sandy soil (1:1) under natural light where they showed normal growth and development.