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Bisphenol A analogues (BPS and BPF) present a greater obesogenic capacity in 3T3-L1 cell line

Author:
Martínez, M.Á., Blanco, J., Rovira, J., Kumar, V., Domingo, J.L., Schuhmacher, M.
Source:
Food and chemical toxicology 2020 v.140 pp. 111298
ISSN:
0278-6915
Subject:
Western blotting, adipocytes, binding proteins, bisphenol A, bisphenol F, bisphenol S, cell lines, cell structures, cell viability, in vitro studies, lipids, oils, peroxisome proliferator-activated receptor gamma, protein synthesis, staining, toxicity, toxicity testing
Abstract:
This study was aimed at comparing the toxicity effects on cell viability and the obesogenic activity of Bisphenol A (BPA) and its analogues, Bisphenol S (BPS) and Bisphenol F (BPF), by in vitro assays with a preadipocytic 3T3-L1 cell line. To compare the toxic potential and select the concentrations of each chemical not showing a decrease in cell viability, MTT assay was performed. The cell phenotype was determined in differentiated 3T3-L1 adipocytes by red oil O staining. To determine the expression levels of the different adipogenic proteins the Western Blot test was performed. The results from MTT assay showed a greater toxic effect of BPA - at equal and even lower concentrations-than its analogues. However, BPS followed by BPF showed a greater neutral lipid storage capacity than BPA, which was reflected in the increase of the protein expression of CCAAT/enhancer binding protein α (C/EBPα), peroxisome proliferator-activated receptor gamma γ (PPARγ) and acid-binding protein 4 (FABP4). In summary, these BPA analogues -especially BPS- present a greater endocrine potential activity than BPA.
Agid:
6873531