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Bisphenol A analogues (BPS and BPF) present a greater obesogenic capacity in 3T3-L1 cell line

Martínez, M.Á., Blanco, J., Rovira, J., Kumar, V., Domingo, J.L., Schuhmacher, M.
Food and chemical toxicology 2020 v.140 pp. 111298
Western blotting, adipocytes, binding proteins, bisphenol A, bisphenol F, bisphenol S, cell lines, cell structures, cell viability, in vitro studies, lipids, oils, peroxisome proliferator-activated receptor gamma, protein synthesis, staining, toxicity, toxicity testing
This study was aimed at comparing the toxicity effects on cell viability and the obesogenic activity of Bisphenol A (BPA) and its analogues, Bisphenol S (BPS) and Bisphenol F (BPF), by in vitro assays with a preadipocytic 3T3-L1 cell line. To compare the toxic potential and select the concentrations of each chemical not showing a decrease in cell viability, MTT assay was performed. The cell phenotype was determined in differentiated 3T3-L1 adipocytes by red oil O staining. To determine the expression levels of the different adipogenic proteins the Western Blot test was performed. The results from MTT assay showed a greater toxic effect of BPA - at equal and even lower concentrations-than its analogues. However, BPS followed by BPF showed a greater neutral lipid storage capacity than BPA, which was reflected in the increase of the protein expression of CCAAT/enhancer binding protein α (C/EBPα), peroxisome proliferator-activated receptor gamma γ (PPARγ) and acid-binding protein 4 (FABP4). In summary, these BPA analogues -especially BPS- present a greater endocrine potential activity than BPA.