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A fast magnetic bead-based colorimetric immunoassay for the detection of tetrodotoxins in shellfish

Campàs, Mònica, Reverté, Jaume, Rambla-Alegre, Maria, Campbell, Katrina, Gerssen, Arjen, Diogène, Jorge
Food and chemical toxicology 2020 v.140 pp. 111315
Crassostrea gigas, Mytilus edulis, Mytilus galloprovincialis, Tetraodontidae, Veneroida, colorimetry, death, foodborne illness, immunoassays, magnetism, mussels, neurotoxins, oysters, poisoning, shellfish, tetrodotoxin, Netherlands
Tetrodotoxin (TTX) is a potent neurotoxin responsible for many food poisoning incidents and some fatalities. Although mainly associated with the consumption of pufferfish, in recent years, TTX has been found in shellfish, particularly in Europe. In this work, a magnetic bead (MB)-based colorimetric immunoassay was applied to the detection of TTX in Pacific oysters (Crassostrea gigas), razor clams (Solen marginatus) and mussels (Mytilus galloprovincialis). Effective LODs (eLODs) for TTX of 1 μg/kg in oysters and razor clams and 3.3 μg/kg in mussels, significantly below the EFSA guidance threshold (44 μg/kg), were obtained. The strategy was applied to the analysis of naturally-contaminated Pacific oysters (Crassostrea gigas) and mussels (Mytilus edulis) from the Netherlands, and TTX was detected in all samples. The approach, which takes less than 1.5 h, proved to be useful as a rapid and simple method to detect TTX, support shellfish safety and protect consumers.