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SWI3B and HDA6 interact and are required for transposon silencing in Arabidopsis
- Yang, Jie, Yuan, Lianyu, Yen, Ming‐Ren, Zheng, Feng, Ji, Rujun, Peng, Tao, Gu, Dachuan, Yang, Songguang, Cui, Yuhai, Chen, Pao‐Yang, Wu, Keqiang, Liu, Xuncheng
- Theplant journal 2020 v.102 no.4 pp. 809-822
- Arabidopsis, DNA methylation, acetylation, eukaryotic cells, gene expression, histone deacetylase, histones, lysine, nucleosomes, transcription (genetics), transposons
- Although the interplay of covalent histone acetylation/deacetylation and ATP‐dependent chromatin remodelling is crucial for the regulation of chromatin structure and gene expression in eukaryotes, the underlying molecular mechanism in plants remains largely unclear. Here we show a direct interaction between Arabidopsis SWI3B, an essential subunit of the SWI/SNF chromatin‐remodelling complex, and the RPD3/HDA1‐type histone deacetylase HDA6 both in vitro and in vivo. Furthermore, SWI3B and HDA6 co‐repress the transcription of a subset of transposons. Both SWI3B and HDA6 maintain transposon silencing by decreasing histone H3 lysine 9 acetylation, but increasing histone H3 lysine 9 di‐methylation, DNA methylation and nucleosome occupancy. Our findings reveal that SWI3B and HDA6 may act in the same co‐repressor complex to maintain transposon silencing in Arabidopsis.