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Vaccination of bovines with recombinant Boophilus Yolk pro-Cathepsin

Author:
Leal, A.T., Seixas, A., Pohl, P.C., Ferreira, C.A.S., Logullo, C., Oliveira, P.L., Farias, S.E., Termignoni, C., Silva Vaz, I. da Jr., Masuda, A.
Source:
Veterinary immunology and immunopathology 2006 v.114 no.3-4 pp. 341-345
ISSN:
0165-2427
Subject:
vaccination, cattle, cattle diseases, recombinant antigens, Boophilus microplus, cathepsins, ova, egg yolk, ticks, aspartic proteinases
Abstract:
Boophilus Yolk pro-Cathepsin (BYC) is an aspartic proteinase found in Boophilus microplus eggs that is involved in the embryogenesis and has been tested as antigen to compose an anti-tick vaccine. The vaccine potential of a recombinant BYC expressed in Escherichia coli (rBYC) was investigated. rBYC was purified and used to immunize Hereford cattle. The sera of bovines immunized with rBYC recognized the native BYC with a titer ranging from 125 to 4000. Furthermore, immunized bovines challenged with 20,000 larvae presented an overall protection of 25.24%. The partial protection obtained against B. microplus infestation with the recombinant protein immunization was similar to the already described for native BYC immunization.
Agid:
698014